PHF1 Antibody (2G7) - Azide and BSA Free

Novus Biologicals | Catalog # H00005252-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2G7

Format

Azide and BSA Free
View all PHF1 Primary Antibodies »

Product Specifications

Immunogen

PHF1 (NP_077084, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AQPPRLSRSGASSLWDPASPAPTSGPRPRLWEGQDVLARWTDGLLYLGTIKKVDSAREVCLVQFEDDSQFLVLWKDISPAALPGEELLCCVCRSETVVP

Specificity

PHF1 (2G7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PHF1 Antibody (2G7) - Azide and BSA Free

Western Blot: PHF1 Antibody (2G7) [H00005252-M04]

Western Blot: PHF1 Antibody (2G7) [H00005252-M04]

Western Blot: PHF1 Antibody (2G7) [H00005252-M04] - PHF1 monoclonal antibody (M04), clone 2G7. Western Blot analysis of PHF1 expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: PHF1 Antibody (2G7) [H00005252-M04]

Immunocytochemistry/ Immunofluorescence: PHF1 Antibody (2G7) [H00005252-M04]

Immunocytochemistry/Immunofluorescence: PHF1 Antibody (2G7) [H00005252-M04] - Analysis of monoclonal antibody to PHF1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: PHF1 Antibody (2G7) [H00005252-M04] - Detection limit for recombinant GST tagged PHF1 is approximately 1ng/ml as a capture antibody.

Applications for PHF1 Antibody (2G7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PHF1

This gene encodes a protein with significant sequence similarity to Drosophila Polycomblike. The encoded protein contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. Two transcript variants have been found for this gene.

Alternate Names

hPCl1, MTF2L2, PCL1, PHD finger protein 1, PHF2, Polycomb-like protein 1, Protein PHF1

Entrez Gene IDs

5252 (Human)

Gene Symbol

PHF1

OMIM

602881 (Human)

UniProt

NP_077084 (Human)

Additional PHF1 Products

Product Documents for PHF1 Antibody (2G7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PHF1 Antibody (2G7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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