PHF5A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88591

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PHF5A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AKHHPDLIFCRKQAGVAIGRLCEKCDGKCVICDSYVRPCTLVRICDECNYGSYQGRCVICGGPGVSDAYYCKECTIQEKDRDGCPKIVNLGSSKTDLFYERK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF5A Antibody - BSA Free

Western Blot: PHF5A Antibody [NBP1-88591]

Western Blot: PHF5A Antibody [NBP1-88591]

Western Blot: PHF5A Antibody [NBP1-88591] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-PHF5A antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: PHF5A Antibody [NBP1-88591]

Immunocytochemistry/ Immunofluorescence: PHF5A Antibody [NBP1-88591]

Immunocytochemistry/Immunofluorescence: PHF5A Antibody [NBP1-88591] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591]

Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591]

Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591]

Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591]

Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591]

Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591]

Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591] - Staining of human cerebral cortex shows strong nuclear positivity in neurons
Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591]

Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591]

Immunohistochemistry-Paraffin: PHF5A Antibody [NBP1-88591] - Staining of human rectum shows strong nuclear positivity in glandular cells.
PHF5A Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: PHF5A Antibody - BSA Free [NBP1-88591]

Chromatin Immunoprecipitation-exo-Seq: PHF5A Antibody - BSA Free [NBP1-88591]

ChIP-Exo-Seq composite graph for Anti-PHF5A (NBP1-88591) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for PHF5A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHF5A

PHF5A encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq]

Alternate Names

bK223H9.2, INI, MGC1346, PHD finger protein 5A, PHD finger-like domain protein 5A, PHD finger-like domain-containing protein 5A, PHD-finger 5a, Rds3, SAP14b, SF3b14bSplicing factor 3B-associated 14 kDa protein, splicing factor 3B associated 14 kDa protein

Gene Symbol

PHF5A

Additional PHF5A Products

Product Documents for PHF5A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PHF5A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PHF5A Antibody - BSA Free

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Protocols

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FAQs

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