PHLDA1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human tonsil shows moderate cytoplasmic positivity in squamous epithelial cells

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells as expected.

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -

Immunohistochemistry-Paraffin: PHLDA1 Antibody [NBP1-84969] -Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Immunohistochemistry: PHLDA1 Antibody [NBP1-84969] -

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Immunocytochemistry/ Immunofluorescence: PHLDA1 Antibody [NBP1-84969] -

Immunocytochemistry/ Immunofluorescence: PHLDA1 Antibody [NBP1-84969] - Localization of PHLDA1 in mouse testes. (a) Detection of PHLDA1 by immunofluorescence (using Ab2, red; DNA stained with DAPI, blue) in testes of a control mouse & subjected to heat shock after 2, 4, & 6 h of recovery. Enlargement of the marked areas is shown in the bottom panel; round spermatids & condensing spermatids are marked with arrowhead & arrows, respectively. Representative pachytene spermatocytes (in squares) are further enlarged in the lower corners. (b) Detection of apoptotic DNA breaks (by TUNEL assay, red; DNA stained with DAPI, blue) in seminiferous tubules of untreated mice & after heat shock in vivo & indicated recovery time (2–6 h). (c) Detection of PHLDA1 by immunofluorescence (red; DNA stained with DAPI, blue) in cryptorchid testes 7 & 14 days after surgery (upper panel) & detection of apoptotic DNA breaks (by TUNEL assay, red) in corresponding tissues (bottom panel). Scale bar—50 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PHLDA1 Antibody [NBP1-84969] -

Western Blot: PHLDA1 Antibody [NBP1-84969] - Expression of pleckstrin-homology-like domain family A, member 1 (PHLDA1) in mouse testes. (a) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in adult testes after heat shock performed in vivo & indicated recovery time. C—control, physiological temperature; HS—heat shock. (b) PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock & indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. (c) Time-line of the appearance of different spermatogenic cells during the mouse postnatal development. (d) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of 11–46-day-old animals. (e) PHLDA1 protein level analyzed by western blot in testes of 11–46-day-old animals. ACTB was used as loading control; two anti-PHLDA1 antibodies (Ab1 & Ab2) were used; heavy chain IgG detected by the secondary anti-mouse antibody is marked with an arrow. (f) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of wild-type (wt) & aHSF1 transgenic (tg) mice at 13th, 15th, 18th, & 21st day of postnatal development (upper panel); fold change in Phlda1 expression quantified by RT-qPCR in testes of tg mice compared to wt mice of the same age (bottom panel; marked are minimum & maximum values). Asterisk indicates the statistical significance of differences (* p < 0.05). (g) PHLDA1 protein level analyzed by western blot in testes of wild-type (wt) & aHSF1 transgenic (tg) mice. ACTB & HSPA8 were used as loading controls. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PHLDA1 Antibody [NBP1-84969] -

Western Blot: PHLDA1 Antibody [NBP1-84969] - Expression of pleckstrin-homology-like domain family A, member 1 (PHLDA1) in mouse testes. (a) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in adult testes after heat shock performed in vivo & indicated recovery time. C—control, physiological temperature; HS—heat shock. (b) PHLDA1 protein level analyzed by western blot in testes of mice subjected to heat shock & indicated recovery time. HSPA8 was used as loading control; proteins were extracted with either RIPA or 2% SDS buffer. (c) Time-line of the appearance of different spermatogenic cells during the mouse postnatal development. (d) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of 11–46-day-old animals. (e) PHLDA1 protein level analyzed by western blot in testes of 11–46-day-old animals. ACTB was used as loading control; two anti-PHLDA1 antibodies (Ab1 & Ab2) were used; heavy chain IgG detected by the secondary anti-mouse antibody is marked with an arrow. (f) Transcripts of Phlda1 & reference genes analyzed by RT-PCR in testes of wild-type (wt) & aHSF1 transgenic (tg) mice at 13th, 15th, 18th, & 21st day of postnatal development (upper panel); fold change in Phlda1 expression quantified by RT-qPCR in testes of tg mice compared to wt mice of the same age (bottom panel; marked are minimum & maximum values). Asterisk indicates the statistical significance of differences (* p < 0.05). (g) PHLDA1 protein level analyzed by western blot in testes of wild-type (wt) & aHSF1 transgenic (tg) mice. ACTB & HSPA8 were used as loading controls. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31906015), licensed under a CC-BY license. Not internally tested by Novus Biologicals.