Phospho-Connexin 43/GJA1 (S368) Antibody

Catalog # Availability Size / Price Qty
Detection of Phospho-Connexin 43/GJA1 (S368) by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Phospho-Connexin 43/GJA1 (S368) Antibody Summary

Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Primate, Zebrafish
This antibody is specific for the 43 kDa Connexin 43/GJA1 in Western blots of rat brain lysates.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide corresponding to amino acid residues surrounding the phospho-S368 of Connexin 43/GJA1
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.


Recommended Concentration
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Phospho-Connexin 43/GJA1 (S368) antibody by Western Blot. View Larger

Detection of Phospho-Connexin 43/GJA1 (S368) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 43 kDa Connexin 43/GJA1 phosphorylated at S368 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 units of lambda phosphatase ( lambda Phosphatase) for 30 minutes before being exposed to the Anti-Phospho-Connexin 43/GJA1 (S368). The immunolabeling of Connexin43 is completely eliminated by treatment with lambda PPase.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.

Background: Connexin 43/GJA1

Connexin 43 (Cx43; also gap junction a-1 protein/GJA1) is a 41-44 kDa member of the connexin family, alpha -type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C‑terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.

  1. Sohl, G. and K. Willecke (2004) Cardiovasc. Res. 62:228.
  2. Willecke, K. et al. (2002) Biol. Chem. 383:725.
  3. Giepmans, B.N.G. (2004) Cardiovasc. Res. 62:233.
  4. Vinken, M. et al. (2006) Cell. Signal. 18:592.
  5. Stout, C. et al. (2004) Curr. Opin. Cell Biol. 16:507.
  6. Bao, X. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C647.
  7. Yogo, K. et al. (2006) J. Reprod. Dev. 52:321.
Entrez Gene IDs
2697 (Human)
Alternate Names
Connexin 43; connexin-43; CX43; CX43gap junction protein, alpha-like; DFNB38; Gap junction 43 kDa heart protein; gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa (connexin 43); gap junction protein, alpha 1, 43kDa; GJA1; GJAL; HSS; ODD; ODDD; ODOD; SDTY3

Product Datasheets

You must select a language.


Citation for Phospho-Connexin 43/GJA1 (S368) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Protein kinase Cdelta-mediated phosphorylation of Connexin43 gap junction channels causes movement within gap junctions followed by vesicle internalization and protein degradation.
    Authors: Cone A, Cavin G, Ambrosi C, Hakozaki H, Wu-Zhang A, Kunkel M, Newton A, Sosinsky G
    J Biol Chem, 2014;289(13):8781-98.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Phospho-Connexin 43/GJA1 (S368) Antibody

There are currently no reviews for this product. Be the first to review Phospho-Connexin 43/GJA1 (S368) Antibody and earn rewards!

Have you used Phospho-Connexin 43/GJA1 (S368) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review