Phospho-Smad3 (S423/S425)/Smad2 (S465/S467) Cell-Based ELISA

Phospho-Smad3 (S423/S425)/Smad2 (S465/S467) Cell-Based ELISA Summary

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

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Preparation and Storage

Background: Smad2/3

Smads are a family of intracellular proteins that mediate signaling by members of the TGF-beta superfamily. Smads are divided into three distinct subgroups based on their different roles in TGF-beta family signal transduction: R-Smads (receptor-regulated), Co-Smads (common partner), and I-Smads (inhibitory). In general, activation of TGF-beta superfamily serine/threonine kinase receptors leads to the phosphorylation of R-Smads (Smads1, 2, 3, 5, and 8). This is followed by recruitment of the Co-Smad (Smad-4) into an R-Smad/Co-Smad complex that translocates to the nucleus to regulate gene expression. I-Smads (Smad6 and Smad7) antagonize this signaling pathway by preventing R-Smads from interacting with the receptor, or by competing with Co-Smads for the generation of R-Smad/Co-Smad complexes.