Phospho-Tyrosine HRP-conjugated Antibody

(4 citations)   
  • Specificity
    Detects endogenous proteins containing phosphorylated tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. Does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.
  • Source
    Monoclonal Mouse IgG1 Clone # 179003
  • Formulation
    Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers and preservatives.
  • Label
    HRP (Horseradish Peroxidase)
  • Western Blot
    Please refer to the PDF datasheet for specifications.
    Please refer to the PDF datasheet for specifications.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Western Blot
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Phospho-Tyrosine
Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.
  • Alternate Names:
    2 amino 3(4 hydroxyphenyl) propanoic acid; 4 hydroxyphenylalanine; PhosphoTyrosine; pTyrosine; Tyrosine
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Claudin-4 controls the receptor tyrosine kinase EphA2 pro-oncogenic switch through ss-catenin.
    Authors: Shang X, Lin X, Howell S
    Cell Commun Signal, 2014;12(1):59.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Increased membrane cholesterol in lymphocytes diverts T-cells toward an inflammatory response.
    Authors: Surls J, Nazarov-Stoica C, Kehl M, Olsen C, Casares S, Brumeanu TD
    PLoS ONE, 2012;7(6):e38733.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts.
    Authors: Sarup J, Jin P, Turin L, Bai X, Beryt M, Brdlik C, Higaki JN, Jorgensen B, Lau FW, Lindley P, Liu J, Ni I, Rozzelle J, Kumari R, Watson SA, Zhang J, Shepard HM
    Mol. Cancer Ther., 2008;7(10):3223-36.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth.
    Authors: Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM
    Biochemistry, 2007;46(7):1799-810.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

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