Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot, ELISA, ELISA Development (Detection)

Label

HRP

Antibody Source

Monoclonal Mouse IgG1 Clone # 179003
View all Phospho-Tyrosine Primary Antibodies »

Product Specifications

Specificity

Detects endogenous proteins containing phosphorylated tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. Does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Phospho-Tyrosine HRP-conjugated Antibody

Detection of Phospho-Tyrosine antibody by Western Blot.

Detection of Phospho-Tyrosine by Western Blot.

Phospho-PDGF R beta ELISA Using HAM1676 as Detection Antibody.

Applications for Phospho-Tyrosine HRP-conjugated Antibody

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers and preservatives.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine HRP-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Phospho-Tyrosine HRP-conjugated Antibody

For research use only

Related Research Areas

GDNF Family

Citations for Phospho-Tyrosine HRP-conjugated Antibody

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FAQs

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