Phospholamban [p Ser16, p Thr17] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-16904

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Phospholamban Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S16 & T17 of human Phospholamban (NP_002658.1). ASTIE

Modification

p Ser16, p Thr17

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Phospholamban [p Ser16, p Thr17] Antibody - BSA Free

Western Blot: Phospholamban [p Ser16, p Thr17] AntibodyAzide and BSA Free [NBP3-16904]

Western Blot: Phospholamban [p Ser16, p Thr17] AntibodyAzide and BSA Free [NBP3-16904]

Western Blot: Phospholamban [p Ser16, p Thr17] Antibody [NBP3-16904] - Western blot analysis of extracts of various cell lines, using Phospholamban antibody (NBP3-16904) at 1:1000 dilution.Hela cells and C6 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for Phospholamban [p Ser16, p Thr17] Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phospholamban

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

Alternate Names

CMD1PPLBcardiac phospholamban, phospholamban

Gene Symbol

PLN

Additional Phospholamban Products

Product Documents for Phospholamban [p Ser16, p Thr17] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Phospholamban [p Ser16, p Thr17] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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