Phosphoserine phosphatase Antibody (001) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90222

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001 expressed in HEK293

Format

Azide and BSA Free
View all Phosphoserine phosphatase Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Phosphoserine phosphatase (Uniprot#: P78330; Met1-Glu225).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Phosphoserine phosphatase Antibody (001) - Azide and BSA Free

Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222]

Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222]

Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Anti-PSPH rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa
Immunoprecipitation: Phosphoserine phosphatase Antibody (001) [NBP2-90222]

Immunoprecipitation: Phosphoserine phosphatase Antibody (001) [NBP2-90222]

Immunoprecipitation: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - PSPH was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg HepG2 Whole Cell Lysate. 0.5 uL anti-PSPH rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PSPH rabbit monoclonal antibody,at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 25 kDa. Observed band size: 25 kDa.
Phosphoserine phosphatase Antibody (001) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody (001) [NBP2-90222] -

Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Immunofluorescence staining of Human PSPH in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90222 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).

Applications for Phosphoserine phosphatase Antibody (001) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phosphoserine phosphatase

Phosphoserine phosphatase is encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq]

Alternate Names

L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, phosphoserine phosphatase, PSPase, PSPEC 3.1.3.3

Gene Symbol

PSPH

Additional Phosphoserine phosphatase Products

Product Documents for Phosphoserine phosphatase Antibody (001) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Phosphoserine phosphatase Antibody (001) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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