PHPT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88804

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PHPT1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHPT1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804]

Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804]

Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804] - Staining of human colon shows strong cytoplasmic positivity of mucus in the goblet cells.
PHPT1 Antibody Immunocytochemistry/Immunofluorescence: PHPT1 Antibody [NBP1-88804]

Immunocytochemistry/Immunofluorescence: PHPT1 Antibody [NBP1-88804]

Staining of human cell line U-251 MG shows localization to nuclear bodies & cytosol.

Applications for PHPT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHPT1

PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek et al., 2002 (PubMed 12383260)).(supplied by OMIM)

Alternate Names

bA216L13.10, DKFZp564M173, EC 3.1.3,14 kDa phosphohistidine phosphatase, EC 3.1.3.-, HSPC141, phosphohistidine phosphatase 11700008C22Rik, phosphohistidine phosphatase 14kDa, PHP14CGI-202, Protein janus-A homolog, RP11-216L13.10, sex-regulated protein janus-a

Gene Symbol

PHPT1

Additional PHPT1 Products

Product Documents for PHPT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PHPT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PHPT1 Antibody - BSA Free

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Protocols

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