PHYHD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81187

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PHYHD1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:VLEGFLSAEECVAMQQRIGEIVAEMDVPLHCRTEFSTQEEEQLRAQGSTDYFLSSGDKIRFFFEKGVFDEKGNFLVPPEKSINKIGHALHAHDPVFKSITHSFKVQTLARSLGLQMPVVV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHYHD1 Antibody - BSA Free

Western Blot: PHYHD1 Antibody [NBP1-81187]

Western Blot: PHYHD1 Antibody [NBP1-81187]

Western Blot: PHYHD1 Antibody [NBP1-81187] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells)
Immunocytochemistry/ Immunofluorescence: PHYHD1 Antibody [NBP1-81187]

Immunocytochemistry/ Immunofluorescence: PHYHD1 Antibody [NBP1-81187]

Immunocytochemistry/Immunofluorescence: PHYHD1 Antibody [NBP1-81187] - Staining of human cell line Hep G2 shows positivity in nucleus but excluded from the nucleoli. Antibody staining shown in green.

Applications for PHYHD1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHYHD1

Probably acts as alpha-ketoglutatare-dependent dioxygenase

Alternate Names

MGC16638, phytanoyl-CoA dioxygenase domain containing 1, phytanoyl-CoA dioxygenase domain-containing protein 1

Gene Symbol

PHYHD1

Additional PHYHD1 Products

Product Documents for PHYHD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PHYHD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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