PIK3CA Antibody (10E5) - BSA Free

Novus Biologicals | Catalog # NBP3-26384

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10E5 expressed in HEK293

Format

BSA Free
View all PIK3CA Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human PIK3CA [UniProt P42336]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIK3CA Antibody (10E5) - BSA Free

PIK3CA Antibody (10E5)

Western Blot: PIK3CA Antibody (10E5) [NBP3-26384] -

Western Blot: PIK3CA Antibody (10E5) [NBP3-26384] - Positive Western Blot detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate.
All lanes: PIK3CA Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 125 kDa
Observed band size: 110 kDa
PIK3CA Antibody (10E5)

Immunocytochemistry/Immunofluorescence: PIK3CA Antibody (10E5) [NBP3-26384] -

Immunocytochemistry/Immunofluorescence: PIK3CA Antibody (10E5) [NBP3-26384] - Staining of Hela Cells with PIK3CA Antibody (10E5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for PIK3CA Antibody (10E5) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: PIK3CA

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found;

Long Name

PI3-Kinase P110 Subunit Alpha

Alternate Names

CLAPO, CLOVE, CWS5, MCMTC, P110alpha, PI3Kalpha

Gene Symbol

PIK3CA

Additional PIK3CA Products

Product Documents for PIK3CA Antibody (10E5) - BSA Free

Certificate of Analysis

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Product Specific Notices for PIK3CA Antibody (10E5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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