PIK3R5 Antibody (OTI4G9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73408

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4G9

Format

Azide and BSA Free
View all PIK3R5 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PIK3R5(NP_055123) produced in HEk293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

97.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIK3R5 Antibody (OTI4G9) - Azide and BSA Free

Western Blot: PIK3R5 Antibody (OTI4G9)Azide and BSA Free [NBP2-73408]

Western Blot: PIK3R5 Antibody (OTI4G9)Azide and BSA Free [NBP2-73408]

Western Blot: PIK3R5 Antibody (OTI4G9) - Azide and BSA Free [NBP2-73408] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3R5.
Immunocytochemistry/ Immunofluorescence: PIK3R5 Antibody (OTI4G9) - Azide and BSA Free [NBP2-73408]

Immunocytochemistry/ Immunofluorescence: PIK3R5 Antibody (OTI4G9) - Azide and BSA Free [NBP2-73408]

Immunocytochemistry/Immunofluorescence: PIK3R5 Antibody (OTI4G9) - Azide and BSA Free [NBP2-73408] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PIK3R5.
Western Blot: PIK3R5 Antibody (OTI4G9)Azide and BSA Free [NBP2-73408]

Western Blot: PIK3R5 Antibody (OTI4G9)Azide and BSA Free [NBP2-73408]

Western Blot: PIK3R5 Antibody (OTI4G9) - Azide and BSA Free [NBP2-73408] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Applications for PIK3R5 Antibody (OTI4G9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PIK3R5

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of 85 kDa (p85) and 110 kDa (p110) subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85alpha and p85beta), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110alpha and p110beta interact with p85alpha, and p110alpha has also been shown to interact with p85beta in vitro. p110delta expression is restricted to white blood cells. It has been shown to bind p85alpha and beta, but it apparently does not phosphorylate these subunits. p110delta seems to have the capacity to autophosphorylate. p110gamma does not interact with the p85 subunits. It has been shown to be activated by alpha and betagamma heterotrimeric G proteins.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 5

Alternate Names

FOAP-2, p101-PI3K

Gene Symbol

PIK3R5

Additional PIK3R5 Products

Product Documents for PIK3R5 Antibody (OTI4G9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PIK3R5 Antibody (OTI4G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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