PIM1 Antibody (ST0513)
Novus Biologicals | Catalog # NBP2-67528
Recombinant Monoclonal Antibody
![Western Blot: PIM1 Antibody (ST0513) [NBP2-67528]](https://resources.rndsystems.com/images/products/PIM1-Antibody-ST0513-Western-Blot-NBP2-67528-img0010.jpg "Western Blot: PIM1 Antibody (ST0513) [NBP2-67528]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # ST0513 expressed in HEK293
Loading...
Product Specifications
Immunogen
Synthetic peptide within human PIM1 aa 20-60. (SwissProt: P11309 Human; SwissProt: P06803 Mouse)
Localization
Cytoplasm, Nucleus, Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PIM1 Antibody (ST0513)
Western Blot: PIM1 Antibody (ST0513) [NBP2-67528]
Western Blot: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of PIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: BT-20 cell lysateLane 2: HepG2 cell lysate Lane 3: SW480 cell lysate![Immunocytochemistry/ Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528]](https://resources.rndsystems.com/images/products/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0009.jpg "Immunocytochemistry/ Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528]")
Immunocytochemistry/ Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528]
Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]](https://resources.rndsystems.com/images/products/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0006.jpg "Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]")
Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]
Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human tonsil tissue using anti-PIM1 antibody. Counter stained with hematoxylin.![Immunocytochemistry/ Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528]](https://resources.rndsystems.com/images/products/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0001.jpg "Immunocytochemistry/ Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528]")
Immunocytochemistry/ Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528]
Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunocytochemistry/ Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528]](https://resources.rndsystems.com/images/products/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0008.jpg "Immunocytochemistry/ Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528]")
Immunocytochemistry/ Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528]
Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]](https://resources.rndsystems.com/images/products/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0002.jpg "Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]")
Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]
Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human breast carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]](https://resources.rndsystems.com/images/products/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0003.jpg "Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]")
Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]
Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human colon cancer tissue using anti-PIM1 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]](https://resources.rndsystems.com/images/products/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0004.jpg "Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]")
Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]
Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]](https://resources.rndsystems.com/images/products/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0005.jpg "Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]")
Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528]
Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human spleen tissue using anti-PIM1 antibody. Counter stained with hematoxylin.Applications for PIM1 Antibody (ST0513)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:400
Western Blot
1:500-1:2000
Application Notes
Use in IHC-Fr reported in scientific literature (PMID:35896115).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PIM1
Long Name
Proviral Integration Site 1
Alternate Names
EC 2.7.11.1, Oncogene PIM1, PIM, pim-1 kinase 44 kDa isoform, pim-1 oncogene, pim-1 oncogene (proviral integration site 1), proto-oncogene serine/threonine-protein kinase pim-1
Gene Symbol
PIM1
Additional PIM1 Products
Product Documents for PIM1 Antibody (ST0513)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PIM1 Antibody (ST0513)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for PIM1 Antibody (ST0513)
Powered by Bioz
Customer Reviews for PIM1 Antibody (ST0513)
There are currently no reviews for this product. Be the first to review PIM1 Antibody (ST0513) and earn rewards!
Have you used PIM1 Antibody (ST0513)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...