PINCH1/LIMS1 Antibody (5G7)

Novus Biologicals | Catalog # NBP2-37581

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G7
View all PINCH1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PINCH1/LIMS1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PINCH1/LIMS1 Antibody (5G7)

Western Blot: PINCH1/LIMS1 Antibody (5G7)BSA Free [NBP2-37581]

Western Blot: PINCH1/LIMS1 Antibody (5G7)BSA Free [NBP2-37581]

Western Blot: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: PINCH1/LIMS1 Antibody (5G7) - BSA Free [NBP2-37581]

Immunocytochemistry/ Immunofluorescence: PINCH1/LIMS1 Antibody (5G7) - BSA Free [NBP2-37581]

Immunocytochemistry/Immunofluorescence: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry: PINCH1/LIMS1 Antibody (5G7) - BSA Free [NBP2-37581]

Flow Cytometry: PINCH1/LIMS1 Antibody (5G7) - BSA Free [NBP2-37581]

Flow Cytometry: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).
Western Blot: PINCH1/LIMS1 Antibody (5G7)BSA Free [NBP2-37581]

Western Blot: PINCH1/LIMS1 Antibody (5G7)BSA Free [NBP2-37581]

Western Blot: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Western blot analysis using PINCH mAb against human PINCH (AA: 87-249) recombinant protein. (Expected MW is 44.2 kDa)
ELISA: PINCH1/LIMS1 Antibody (5G7) - BSA Free [NBP2-37581]

ELISA: PINCH1/LIMS1 Antibody (5G7) - BSA Free [NBP2-37581]

ELISA: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PINCH1/LIMS1 Antibody (5G7)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PINCH1

The protein encoded by the LIMS1 gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. (provided by RefSeq)

Long Name

Particularly Interesting New Cys-His Protein 1

Alternate Names

LIMS1

Gene Symbol

LIMS1

Additional PINCH1 Products

Product Documents for PINCH1/LIMS1 Antibody (5G7)

Certificate of Analysis

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Product Specific Notices for PINCH1/LIMS1 Antibody (5G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Integrins and Associated Molecules

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Protocols

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FAQs

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