PKA C alpha Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99335

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all PKA C alpha Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PKA C alpha.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PKA C alpha Antibody - Azide and BSA Free

Western Blot: PKA C alpha Antibody [NBP2-99335]

Western Blot: PKA C alpha Antibody [NBP2-99335]

Western Blot: PKA C alpha Antibody [NBP2-99335] - Anti-PKA C alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa
Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335]

Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335]

Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335]

Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335]

Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

Applications for PKA C alpha Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:400

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKA C alpha

PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit alpha

Alternate Names

Cs, Pkaca, PKCA1, PKCD, PRKACA

Gene Symbol

PRKACA

Additional PKA C alpha Products

Product Documents for PKA C alpha Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PKA C alpha Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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