PKC theta Antibody (M217)

Name: PKC theta Antibody (M217)
Brand: Novus Biologicals
SKU: NBP3-23308
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23308

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # M217

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Product Specifications

Immunogen

Clone (M217) was generated from a recombinant mouse PKC theta protein that included amino acids residues in the N-terminal region. This sequence is conserved in human and rat PKC theta, and has low homology to other PKC family members. [UniProt# A6H667]

Specificity

This antibody detects a 79 kDa protein corresponding to the molecular mass of PKC theta on SDS-PAGE immunoblots of human Jurkat cell lysates.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for PKC theta Antibody (M217)

Western Blot: PKC theta Antibody (M217) [NBP3-23308] -

Western Blot: PKC theta Antibody (M217) [NBP3-23308] - Western blot analysis of PKC theta in human Jurkat cell lysate. The blot was probed with anti-PKC theta at 1:250 (lane 1) and 1:1000 (lane 2).

Immunocytochemistry/Immunofluorescence: PKC theta Antibody (M217) [NBP3-23308] -

Immunocytochemistry/Immunofluorescence: PKC theta Antibody (M217) [NBP3-23308] - Immunocytochemical labeling of PKC theta in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal PKC theta (N-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.

Applications for PKC theta Antibody (M217)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: PKC theta

Members of the protein kinase C (PKC) family play a key regulatory role in variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, episilon, zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and episilon are independent of Ca++. On the other hand, nPKC and episilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Long Name

Protein Kinase C theta

Alternate Names

PRKCQ, PRKCT

Gene Symbol

PRKCQ

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Product Specific Notices for PKC theta Antibody (M217)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.