PKN1 Antibody (1B10) - Azide and BSA Free

Novus Biologicals | Catalog # H00005585-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1B10

Format

Azide and BSA Free
View all PKN1 Primary Antibodies »

Product Specifications

Immunogen

PKN1 (AAH40061, 462 a.a. ~ 615 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDF

Specificity

PKN1 - protein kinase N1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PKN1 Antibody (1B10) - Azide and BSA Free

Western Blot: PKN1 Antibody (1B10) [H00005585-M01]

Western Blot: PKN1 Antibody (1B10) [H00005585-M01]

Western Blot: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 expression in transfected 293T cell line by PKN1 monoclonal antibody (M01), clone 1B10.Lane 1: PKN1 transfected lysate(103.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PKN1 Antibody (1B10) [H00005585-M01]

Immunocytochemistry/ Immunofluorescence: PKN1 Antibody (1B10) [H00005585-M01]

Immunocytochemistry/Immunofluorescence: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of monoclonal antibody to PKN1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PKN1 Antibody (1B10) [H00005585-M01]

Western Blot: PKN1 Antibody (1B10) [H00005585-M01]

Western Blot: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 over-expressed 293 cell line, cotransfected with PKN1 Validated Chimera RNAi ( Cat # H00005585-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PKN1 monoclonal antibody (M01) clone 1B10 (Cat # H00005585-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunoprecipitation: PKN1 Antibody (1B10) [H00005585-M01]

Immunoprecipitation: PKN1 Antibody (1B10) [H00005585-M01]

Immunoprecipitation: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 transfected lysate using anti-PKN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PKN1 monoclonal antibody.
ELISA: PKN1 Antibody (1B10) [H00005585-M01]

ELISA: PKN1 Antibody (1B10) [H00005585-M01]

ELISA: PKN1 Antibody (1B10) [H00005585-M01] - Detection limit for recombinant GST tagged PKN1 is approximately 3ng/ml as a capture antibody.

Applications for PKN1 Antibody (1B10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKN1

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Long Name

Serine/Threonine Protein Kinase N1

Alternate Names

DBK, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1

Entrez Gene IDs

5585 (Human)

Gene Symbol

PKN1

OMIM

601032 (Human)

UniProt

AAH40061 (Human)

Additional PKN1 Products

Product Documents for PKN1 Antibody (1B10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PKN1 Antibody (1B10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Epigenetics
Intracellular Kinases

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Protocols

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FAQs

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