PKR [p Thr451] Antibody (JE46-70)

Western Blot: PKR [p Thr451] Antibody (JE46-70) [NBP3-32789]

Western Blot: PKR [p Thr451] Antibody (JE46-70) [NBP3-32789] - Western blot analysis of PKR on different lysates with Rabbit anti-PKR antibody (NBP3-32789) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10ng/mL IFN-a1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate
Lane 3: HeLa treated with 10ng/mL IFN-a1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with pp for 1 hour

Lysates/proteins at 20 ug/Lane.

Predicted band size: 62 kDa
Observed band size: 70 kDa

Exposure time: 3 minutes 5 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32789) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: PKR [p Thr451] Antibody (JE46-70) [NBP3-32789]

Flow Cytometry: PKR [p Thr451] Antibody (JE46-70) [NBP3-32789] - Flow cytometric analysis of HeLa cells treated with 10ng/mL IFN-alpha 1 for 18 hours then add 100nM Calyculin A for 15 minutes labeling PKR.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32789, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).