Secretory phopholipase A2 (PLA2) enzymes cleave an acyl ester bond in the sn-2 position of glycerophospholipids. These extracellular proteins have a high disulfide bond content, low molecular mass (14 kDa), and require mM levels of Ca2+ for catalysis. They play a crucial role in the generation of arachidonates and eicosanoids, and have a number of biological actions including immunological responses, inflammation, cellular proliferation, vasoconstriction, and bronchioconstriction.
PLA2G4A Antibody (C19) - BSA Free
Novus Biologicals | Catalog # NBP3-41481
Loading...
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # C19
Format
BSA Free
Loading...
Product Specifications
Immunogen
Immunized with recombinant Human PLA2G4A (Uniprot#: P47712; Met1-Ser178 (Uniprot#: P47712)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Novus Biologicals Mouse PLA2G4A Antibody (C19) - BSA Free (NBP3-41481) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PLA2G4A Antibody (C19) - BSA Free
Western Blot: PLA2G4A Antibody (C19) [NBP3-41481] -
Western Blot: PLA2G4A Antibody (C19) [NBP3-41481] - Sample: Recombinant PLA2G4A, Human.
Western Blot: PLA2G4A Antibody (C19) [NBP3-41481] -
Western Blot: PLA2G4A Antibody (C19) [NBP3-41481] - Sample: Human Lung lysate Primary Ab: 1ug/ml NBP3-41481; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Western Blot: PLA2G4A Antibody (C19) [NBP3-41481] -
Western Blot: PLA2G4A Antibody (C19) [NBP3-41481] - Sample: Hela cell lysate Primary Ab: 1ug/ml NBP3-41481; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal AntibodyApplications for PLA2G4A Antibody (C19) - BSA Free
Application
Recommended Usage
Immunohistochemistry
5-30ug/mL
Immunohistochemistry-Paraffin
5-30ug/mL
Western Blot
0.5-3ug/mL
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01M PBS, pH7.4, 50% glycerol
Format
BSA Free
Preservative
0.05% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PLA2G4A
Long Name
Phospholipase A2 Group IV A
Alternate Names
cPLA2, cPLA2-alpha
Gene Symbol
PLA2G4A
Additional PLA2G4A Products
Product Documents for PLA2G4A Antibody (C19) - BSA Free
Product Specific Notices for PLA2G4A Antibody (C19) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for PLA2G4A Antibody (C19) - BSA Free
There are currently no reviews for this product. Be the first to review PLA2G4A Antibody (C19) - BSA Free and earn rewards!
Have you used PLA2G4A Antibody (C19) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for PLA2G4A Antibody (C19) - BSA Free
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: Hi. Actually I want to purchase anti cPLA2 antibody raised in rabbit which can bind to both phorphorylated and unphosphorylated mouse cPLA2 group IV. I want to a gel shift assay with western blotting to show that my protein of interest is causing the phosphorylation of cPLA2 inside the mouse macrophages RAW cells.Can you please tell me which antibody would be suitable for the purpose. Thanks a lot.
A: Here are our antibodies raised in rabbit that have been validated to detect cPLA2 in mouse samples in a western blot. We have not specifically confirmed the ability of these antibodies to detect both the phosphorylated and unphosphorylated forms of the protein, and I cannot guarantee that they will clearly differentiate between the two.
Loading...
Associated Pathways
