PLC-gamma 1 Antibody - BSA Free

Name: PLC-gamma 1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-38316
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-38316

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLC-gamma 1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PLC-gamma 1 Antibody [NBP2-38316]

Immunocytochemistry/Immunofluorescence: PLC-gamma 1 Antibody [NBP2-38316] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Immunohistochemistry: PLC-gamma 1 Antibody [NBP2-38316]

Immunohistochemistry: PLC-gamma 1 Antibody [NBP2-38316] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.

Applications for PLC-gamma 1 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLC-gamma 1

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups (1). Phospholipase C gamma 1 (PLC-gamma 1) hydrolyses phosphatidylinositol-4,5-bisphosphate to the second messengers inositol-1,4,5-trisphosphate and diacylglycerol. PLC-gamma 1 also has mitogenic activity upon growth-factor-dependent tyrosine phosphorylation; however, this activity is not dependent on the phospholipase activity of PLC-gamma 1, but requires an SH3 domain (2). Also, results indicate a lipase-independent role of PLC-gamma in the physiological agonist-induced activation of Ca2+ entry (3).

Long Name

Phospholipase C gamma 1

Alternate Names

PLCG1, PLCgamma 1, PPLCA

Gene Symbol

PLCG1

UniProt

P19174

Additional PLC-gamma 1 Products

Product Documents for PLC-gamma 1 Antibody - BSA Free

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Product Specific Notices for PLC-gamma 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.