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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Plexin A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the N-terminal half of the semaphorin domain in human Plexin A1. This sequence is highly conserved in rat and mouse Plexin A1, and has low homology to other Plexin family members. [UniProt# Q9UIW2]
Specificity
The antibody detects a 220 kDa protein corresponding to the apparent molecular mass of Plexin A1 on SDS-PAGE immunoblots of neonatal rat brain and Cos-7 cells transfected with mouse Plexin A1. In immunocytochemistry, this antibody co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin A1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Plexin A1 Antibody
Western Blot: Plexin A1 Antibody [NBP3-23309] -
Western Blot: Plexin A1 Antibody [NBP3-23309] - Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin A1 immunoprecipitated from Myc-tagged Plexin A1 transfected cells using anti-Myc (lane 4) or anti-Plexin A1 (NBP3-23309; lane 5). These blots were probed with either the affinity purified anti-Plexin A1 (NBP3-23309; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).
Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP3-23309] -
Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP3-23309] - Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin A1 rabbit polyclonal (NBP3-23309) antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin A1 peptide in the presence of this anti-Plexin A1 antibody (C,F).Applications for Plexin A1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:100
Immunoprecipitation
1:50
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Plexin A1
Alternate Names
NOVP, PLXN1, PLXNA1
Gene Symbol
PLXNA1
Additional Plexin A1 Products
Product Documents for Plexin A1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Plexin A1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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