PLGF Antibody (37203.111) - Azide and BSA Free

Novus Biologicals | Catalog # NB120-10966

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 37203.111

Format

Azide and BSA Free
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Product Specifications

Immunogen

Purified recombinant human placenta growth factor expressed in E. coli.

Localization

The three isoforms are secreted but PlGF2 appears to remain cell attached unless released by heparin.

Specificity

In immunoblotting, the shows no cross-reactivity with recombinanthuman VEGF, PDGFAA, PDGFAB, and PDGFBB.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for PLGF Antibody (37203.111) - Azide and BSA Free

Application
Recommended Usage

ELISA

2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: For capture ELISAs, this product can be used as the capture antibody. Use at a working concentration in combination with a biotinylated PlGF polyclonal detection antibody at 100 ng/ml (100 ul/well). WB: The detection limit for recombinant human placental growth factor is approximately 20 ng/lane and 300 ng/lane under non-reducing and reducing conditions, respectively. Homodimeric recombinant PlGF migrates as an approximately 29 kDa protein.

Reviewed Applications

Read 1 review rated 3 using NB120-10966 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS and carbohydrates

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PlGF

PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It belongs to the cysteine-knot superfamily. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor.

Long Name

Placenta Growth Factor

Alternate Names

PGF, PGFL

Entrez Gene IDs

5228 (Human)

Gene Symbol

PGF

UniProt

P49763

Additional PlGF Products

Product Documents for PLGF Antibody (37203.111) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PLGF Antibody (37203.111) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PLGF Antibody (37203.111) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: ELISA
    Sample Tested: PLGF recombinant protein
    Species: Human
    Verified Customer | Posted 02/18/2009

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FAQs for PLGF Antibody (37203.111) - Azide and BSA Free

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  • Q:

    Does the Human PlGF Quantikine® ELISA Kit, Catalog # DPG00, detect free or total PlGF?

    A:

    Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. We cannot claim Catalog # DPG00 to be a "free" or "total" PlGF detecting kit, since all possible binding factors have not been tested. 

    Specificity testing performed using rhFlt-1/Fc (VEGF R1) for interference demonstrated interference at levels greater than 2000 pg/mL. Details of this testing can be found in the DPG00 product insert. Most samples will have lower levels of sFlt-1 than 2000 pg/ml.

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