PLK1 Antibody (OTI1D4)

Novus Biologicals | Catalog # NBP1-48291

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1D4
View all PLK1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PLK1 (NP_005021) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Mitosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

68.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLK1 Antibody (OTI1D4)

Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291]

Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291]

Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded human endometrium tissue using PLK1 antibody (OTI1D4). Primary antibody dilution 1:200. Image from verified customer review.
Flow Cytometry: PLK1 Antibody (OTI1D4) [NBP1-48291]

Flow Cytometry: PLK1 Antibody (OTI1D4) [NBP1-48291]

Flow Cytometry: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells transfected with either PLK1 Human Tagged ORF Clone overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-PLK1 antibody, and then analyzed by flow cytometry (1:100).
Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291]

Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291]

Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291]

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human prostate tissue using anti-PLK1 mouse monoclonal antibody.

Applications for PLK1 Antibody (OTI1D4)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 5 using NBP1-48291 in the following applications:

Flow Cytometry Panel Builder

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  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

2.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PLK1

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Entrez Gene IDs

5347 (Human)

Gene Symbol

PLK1

UniProt

P53350

Additional PLK1 Products

Product Documents for PLK1 Antibody (OTI1D4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PLK1 Antibody (OTI1D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

Customer Reviews for PLK1 Antibody (OTI1D4) (1)

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  • PLK1 Antibody (OTI1D4)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Endometrium
    Species: Mouse
    Verified Customer | Posted 12/18/2021
    Staining in endometrium
    Primary antibody dilution 1:200, secondary antibody 1:300
    PLK1 Antibody (OTI1D4) NBP1-48291

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Protocols

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