PLK1 Antibody (OTI1D4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73472

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1D4

Format

Azide and BSA Free
View all PLK1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PLK1 (NP_005021) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

68.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLK1 Antibody (OTI1D4) - Azide and BSA Free

Western Blot: PLK1 Antibody (OTI1D4)Azide and BSA Free [NBP2-73472]

Western Blot: PLK1 Antibody (OTI1D4)Azide and BSA Free [NBP2-73472]

Western Blot: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody.
Flow Cytometry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Flow Cytometry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Flow Cytometry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - HEK293T cells transfected with either PLK1 Human Tagged ORF Clone overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostaining by anti-PLK1 antibody, and then analyzed by flow cytometry
Western Blot: PLK1 Antibody (OTI1D4)Azide and BSA Free [NBP2-73472]

Western Blot: PLK1 Antibody (OTI1D4)Azide and BSA Free [NBP2-73472]

Western Blot: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of
Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472]

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Human prostate tissue using anti-PLK1 mouse monoclonal antibody.

Applications for PLK1 Antibody (OTI1D4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLK1

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Gene Symbol

PLK1

Additional PLK1 Products

Product Documents for PLK1 Antibody (OTI1D4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PLK1 Antibody (OTI1D4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

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Protocols

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