Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PLK1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residues 325 and 375 of human Polo-like kinase using the numbering given in SwissProt entry P53350 (GeneID 5347).

Marker

Mitosis Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLK1 Antibody - BSA Free

Western Blot: PLK1 Antibody [NB100-547]

Western Blot: PLK1 Antibody [NB100-547]

Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody NB100-547 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunocytochemistry/ Immunofluorescence: PLK1 Antibody [NB100-547]

Immunocytochemistry/ Immunofluorescence: PLK1 Antibody [NB100-547]

Immunocytochemistry/Immunofluorescence: PLK1 Antibody [NB100-547] - Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- PLK1 (NB100-547). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.
Western Blot: PLK1 Antibody [NB100-547]

Western Blot: PLK1 Antibody [NB100-547]

Western Blot: PLK1 Antibody [NB100-547] - Detection of Human PLK1 on HeLa whole cell lysate using NB100-547. B. Affinity purified rabbit anti-PLK1 antibodies NB100-546, NB100-547, and NB100-548. IPed PLK1 was detected using NB100-548.

Applications for PLK1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2000

Immunoprecipitation

2-4 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PLK1

Polo-like kinases are a family of serine/threonine protein kinases, named after the prototypic founding member of the family, the polo gene product of Drosophila melanogaster. The polo kinase was originally identified in mutants that display abnormal mitotic spindle organization. Subsequently, potential homologues of Drosophila polo have been identified in yeasts (Cdc5p in Saccharomyces cerevisiae; plo1+ in Schizosaccharmoyces pombe) and in mammals (polo-like kinase 1; Plk1). Genetic and biochemical studies suggest that polo, Cdc5p and plo1+ may be required for mitotic spindle organization and, possibly, for cytokinesis. Likewise, the patterns of expression, activity and subcellular localization of Plk1 suggest that Plk1 may function during mitosis in spindle assembly and function. Recent studies have demonstrated that mammalian Plks regulate the function of the Golgi complex, a cellular organelle closely associated with the centrosome and also have microtubule organization activity. Furthermore, deregulated expression of human PLK1 and PLK3 is strongly correlated with the development of many types of malignancies, and ectopic expression of kinase-active Plk3 or Plk1 dominant negative protein leads to rapid cell death.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Entrez Gene IDs

5347 (Human); 18817 (Mouse)

Gene Symbol

PLK1

UniProt

P53350

Additional PLK1 Products

Product Documents for PLK1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PLK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

Citations for PLK1 Antibody - BSA Free

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Protocols

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