PLK1 Antibody

Novus Biologicals | Catalog # NB100-56651

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (99%), Mouse (97%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PLK1 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 100-200 of human PLK1 was used as the immunogen.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (87%).

Marker

Mitosis Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLK1 Antibody

Western Blot: PLK1 Antibody [NB100-56651]

Western Blot: PLK1 Antibody [NB100-56651]

Western Blot: PLK1 Antibody [NB100-56651] - Analysis of HCT-116 lysate using PLK1 antibody at 1:2000.
Immunohistochemistry-Paraffin: PLK1 Antibody [NB100-56651]

Immunohistochemistry-Paraffin: PLK1 Antibody [NB100-56651]

Immunohistochemistry-Paraffin: PLK1 Antibody [NB100-56651] - Analysis of human placenta using PLK1 antibody at 1:100.

Applications for PLK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 28474672)

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera with PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLK1

Polo related kinase (PLK), a serine/threonine kinase is strongly correlated with the mitotic activity of cells. PLK is present in all tissues during mouse embryonic development, where as in adults expresion is limited to thymus and ovaries. It has been observed that tumors of various origin (lung, colon, stomach, smooth muscle, and esophagus as well as non-Hodgkin lymphomas) express high levels of PLK transcripts with absent of transcripts in normal surrounding tissues. Only normal colon and placenta, which are known to be mitotically active express PLK. PLK expression is restricted to proliferating cells. In mammalian cells, three PLKs (PLK1, PLK2/Snk, PLK3/Fnk) have been identified. PLK1 is the most studied isoform. The known substrates of PLK1 include several proteins involved in mitosis such as Cdc25C, APC, and cyclin B. PLK1 promotes mitosis through the phosphorylation and activation of Cdc25C, which regulates the tyrosine dephosphorylation of CDKs.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Entrez Gene IDs

18817 (Mouse); 25515 (Rat)

Gene Symbol

PLK1

UniProt

P53350

Additional PLK1 Products

Product Documents for PLK1 Antibody

Certificate of Analysis

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Product Specific Notices for PLK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

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Protocols

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