PMP70 Antibody (CL2524)

Novus Biologicals | Catalog # NBP3-52459

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL2524
View all PMP70 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P28288, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PMP70 Antibody (CL2524)

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (CL2524) [NBP3-52459]

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (CL2524) [NBP3-52459]

Staining in A431 cell line showing specific staining of peroxisomes in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (CL2524) [NBP3-52459]

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (CL2524) [NBP3-52459]

Staining in HeLa cell line showing specific staining of peroxisomes in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (CL2524) [NBP3-52459]

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (CL2524) [NBP3-52459]

Staining in MCF7 cell line showing specific staining of peroxisomes in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (CL2524) [NBP3-52459]

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (CL2524) [NBP3-52459]

Staining in U251 cell line showing specific staining of peroxisomes in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (CL2524) [NBP3-52459]

Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (CL2524) [NBP3-52459]

Staining in U2OS cell line showing specific staining of peroxisomes in green. Microtubule and nuclear probes are visualized in red and blue respectively (where available).

Applications for PMP70 Antibody (CL2524)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PMP70

PMP70 is encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Alternate Names

70 kDa peroxisomal membrane protein, ABC43, ATP-binding cassette, sub-family D (ALD), member 3, peroxisomal membrane protein 1 (70kD, Zellweger syndrome), Peroxisomal membrane protein-1 (70kD), PMP70ATP-binding cassette sub-family D member 3, PXMP1dJ824O18.1 (ATP-binding cassette, sub-family D (ALD), member 3 (PMP70, PXMP1)), ZWS2

Gene Symbol

ABCD3

Additional PMP70 Products

Product Documents for PMP70 Antibody (CL2524)

Certificate of Analysis

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Product Specific Notices for PMP70 Antibody (CL2524)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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