PNMT Antibody (CL11434) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44139

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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL11434

Format

Azide and BSA Free
View all PNMT Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P11086, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PNMT Antibody (CL11434) - Azide and BSA Free

PNMT Antibody (CL11434) - Azide and BSA Free Immunohistochemistry-Paraffin: PNMT Antibody [NBP3-44139]

Immunohistochemistry-Paraffin: PNMT Antibody [NBP3-44139]

Analysis in human adrenal gland and kidney tissues using NBP3-44139 antibody. Corresponding PNMT RNA-seq data are presented for the same tissues.
PNMT Antibody (CL11434) - Azide and BSA Free Immunohistochemistry-Paraffin: PNMT Antibody [NBP3-44139]

Immunohistochemistry-Paraffin: PNMT Antibody [NBP3-44139]

Staining of human Adrenal gland shows strong cytoplasmic positivity in neuroendocrine cells.
PNMT Antibody (CL11434) - Azide and BSA Free Immunohistochemistry-Paraffin: PNMT Antibody [NBP3-44139]

Immunohistochemistry-Paraffin: PNMT Antibody [NBP3-44139]

Staining of human Cerebral cortex shows very weak cytoplasmic positivity in neuronal cells.
PNMT Antibody (CL11434) - Azide and BSA Free Immunohistochemistry-Paraffin: PNMT Antibody [NBP3-44139]

Immunohistochemistry-Paraffin: PNMT Antibody [NBP3-44139]

Staining of human Duodenum shows no positivity in glandular cells as expected.
PNMT Antibody (CL11434) - Azide and BSA Free Immunohistochemistry-Paraffin: PNMT Antibody [NBP3-44139]

Immunohistochemistry-Paraffin: PNMT Antibody [NBP3-44139]

Staining of human Kidney shows no positivity in cells in seminiferous ducts as expected.

Applications for PNMT Antibody (CL11434) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PNMT

The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production.

Long Name

Phenylethanolamine N-Methyltransferase

Alternate Names

Noradrenaline N-methyltransferase, PENT, PNMTase

Gene Symbol

PNMT

Additional PNMT Products

Product Documents for PNMT Antibody (CL11434) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PNMT Antibody (CL11434) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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