PNPO Antibody (OTI1G9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73509

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1G9

Format

Azide and BSA Free
View all PNPO Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PNPO(NP_060599) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PNPO Antibody (OTI1G9) - Azide and BSA Free

Western Blot: PNPO Antibody (OTI1G9)Azide and BSA Free [NBP2-73509]

Western Blot: PNPO Antibody (OTI1G9)Azide and BSA Free [NBP2-73509]

Western Blot: PNPO Antibody (OTI1G9) - Azide and BSA Free [NBP2-73509] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO.
Flow Cytometry: PNPO Antibody (OTI1G9) - Azide and BSA Free [NBP2-73509]

Flow Cytometry: PNPO Antibody (OTI1G9) - Azide and BSA Free [NBP2-73509]

Flow Cytometry: PNPO Antibody (OTI1G9) - Azide and BSA Free [NBP2-73509] - Analysis of Jurkat cells, using PNPO antibody,(Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: PNPO Antibody (OTI1G9) - Azide and BSA Free [NBP2-73509]

Flow Cytometry: PNPO Antibody (OTI1G9) - Azide and BSA Free [NBP2-73509]

Flow Cytometry: PNPO Antibody (OTI1G9) - Azide and BSA Free [NBP2-73509] - Analysis of Hela cells, using PNPO antibody (Red), compared to a nonspecific negative control antibody (Blue).

Applications for PNPO Antibody (OTI1G9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PNPO

Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. The rate-limiting enzyme in vitamin B6 synthesis is pyridoxine-5-prime-phosphate (PNP) oxidase (PNPO; EC 1.4.3.5).[supplied by OMIM]

Alternate Names

EC 1.4.3.5, FLJ10535, PDXPO, pyridoxal 5'-phosphate synthase, pyridoxamine 5'-phosphate oxidase, Pyridoxamine-phosphate oxidase, pyridoxine 5'-phosphate oxidase, pyridoxine-5'-phosphate oxidase

Gene Symbol

PNPO

Additional PNPO Products

Product Documents for PNPO Antibody (OTI1G9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PNPO Antibody (OTI1G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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