Podoplanin Antibody (18H5) - Azide and BSA Free

Novus Biologicals | Catalog # NB600-1013

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 18H5

Format

Azide and BSA Free
View all Podoplanin Primary Antibodies »

Product Specifications

Immunogen

MDCK cells stably expressing gp36 (Podoplanin) protein

Localization

Cell Membrane

Marker

Lymphatic Endothelium Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Podoplanin Antibody (18H5) - Azide and BSA Free

Western Blot: Podoplanin Antibody (18H5)Azide and BSA Free [NB600-1013]

Western Blot: Podoplanin Antibody (18H5)Azide and BSA Free [NB600-1013]

Western Blot: Podoplanin Antibody (18H5) [NB600-1013] - Fig. 1: Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis using our monoclonal and polyclonal antibodies. Both antibodies recognize a protein of about 36 kDa in total lysate from LECs but not from HUVEC.
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (18H5) - Azide and BSA Free [NB600-1013]

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (18H5) - Azide and BSA Free [NB600-1013]

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (18H5) [NB600-1013] - staining (green) of Podoplanin in primary human dermal lymphatic endothelial cells (HDLEC) with anti-human Podoplanin (0,5ug/ml) and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 was used 1:400.
Flow Cytometry: Podoplanin Antibody (18H5) - Azide and BSA Free [NB600-1013]

Flow Cytometry: Podoplanin Antibody (18H5) - Azide and BSA Free [NB600-1013]

Flow Cytometry: Podoplanin Antibody (18H5) [NB600-1013] - analysis with human primary lymphatic endothelial cells (HDLEC). As secondary antibody anti-mouse IgG-PE was used.
Flow Cytometry: Podoplanin Antibody (18H5) - Azide and BSA Free [NB600-1013]

Flow Cytometry: Podoplanin Antibody (18H5) - Azide and BSA Free [NB600-1013]

Flow Cytometry: Podoplanin Antibody (18H5) [NB600-1013] - Fig. 1: Flow analysis of Podoplanin expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC). As expected there is no expression of Podoplanin detectable in the HUVECs but a strong one in the HDMVEC.

Applications for Podoplanin Antibody (18H5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 - 10 ug/ml

Immunocytochemistry/ Immunofluorescence

6 - 30 ug/ml

Immunohistochemistry

6 - 30 ug/ml

Western Blot

1 - 5 ug/ml
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Podoplanin

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (18H5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Podoplanin Antibody (18H5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Podoplanin Antibody (18H5) - Azide and BSA Free

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Protocols

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FAQs

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