Podoplanin Antibody (PDPN/1433)

Detection of Podoplanin in Human Lung via seqIF™ staining on COMET™

Podoplanin was detected in immersion fixed paraffin-embedded sections of human Lung using Mouse Anti-Human Podoplanin, Monoclonal Antibody (Catalog # NBP2-53292) at 0.5ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/1433) [NBP2-53292]

Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/1433) [NBP2-53292] - Formalin-fixed, paraffin-embedded human Cervix stained with Podoplanin Monoclonal Antibody (PDPN/1433)

Protein Array: Podoplanin Antibody (PDPN/1433) [NBP2-53292]

Protein Array: Podoplanin Antibody (PDPN/1433) [NBP2-53292] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Podoplanin Antibody (PDPN/1433) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.