Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free

Name: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-11971
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-11971

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Block/Neutralize, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # PMab-1

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.

Specificity

This antibody is specific for amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin (PDPN). This antibody, therefore, possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), which is derived from the PLAG domain of murine podoplanin.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free

Flow Cytometry: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971]

Flow Cytometry: Podoplanin Antibody (PMab-1) - Chimeric [NBP3-11971] - NIH3T3 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of PMab-1 (NBP3-11971, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western Blot: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] -

Western Blot: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Podoplanin (MAP tag) expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP3-11971 at 0.00001ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunocytochemistry /Immunofluorescence: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] -

Immunocytochemistry /Immunofluorescence: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with Podoplanin (MAP tag) expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with NBP3-11971 (1:200 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).

Western Blot: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] -

Western Blot: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with NBP3-11971 at 0.01 ug/ml, 0.1 ug/ml, 0.001 ug/ml and 0.003 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

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