Key Product Details

Species Reactivity

Mouse, Human (Negative)

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG1 Clone # RTD4E10

Format

Azide and BSA Free
View all Podoplanin Primary Antibodies »

Product Specifications

Immunogen

Murine thymic stromal cell line

Clonality

Monoclonal

Host

Hamster

Isotype

IgG1

Description

Novus Biologicals Hamster Podoplanin Antibody (RTD4E10) - Azide and BSA Free (NBP2-36058) is a monoclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Podoplanin Antibody (RTD4E10) - Azide and BSA Free

Western Blot: Podoplanin Antibody (RTD4E10)Azide and BSA Free [NBP2-36058]

Western Blot: Podoplanin Antibody (RTD4E10)Azide and BSA Free [NBP2-36058]

Western Blot: Podoplanin Antibody (RTD4E10) [NBP2-36058] - Analysis with recombinant human, mouse and ratsoluble Podoplanin. There is no cross reaction with human Podoplanin.
ELISA: Podoplanin Antibody (RTD4E10) - Azide and BSA Free [NBP2-36058]

ELISA: Podoplanin Antibody (RTD4E10) - Azide and BSA Free [NBP2-36058]

ELISA: Podoplanin Antibody (RTD4E10) [NBP2-36058] - Functional ELISA with anti-mouse Podoplanin. Recombinant mouse soluble Podoplanin was coated with increasing amounts in a 96 well microtiter plate.

Applications for Podoplanin Antibody (RTD4E10) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1-5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Podoplanin

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (RTD4E10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Podoplanin Antibody (RTD4E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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