Polycystin-1/PKD1 Antibody

Novus Biologicals | Catalog # NBP1-52552

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Polycystin-1/PKD1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide C-RTPLRAKNKVHP from the C-terminus of human Polycystin-1/PKD1 (NP_001009944.1; NP_000287.2).

Epitope

C-RTPLRAKNKVHP from the C-Terminus

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (83%), Feline (83%), Canine (83%), Equine (83%).

Specificity

Human PKD1. This antibody is expected to recognize both reported isoforms (NP_001009944.1 and NP_000287.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Polycystin-1/PKD1 Antibody

Immunohistochemistry-Paraffin: Polycystin-1/PKD1 Antibody [NBP1-52552]

Immunohistochemistry-Paraffin: Polycystin-1/PKD1 Antibody [NBP1-52552]

Immunohistochemistry-Paraffin: Polycystin-1/PKD1 Antibody [NBP1-52552] - Analysis of anti-PKD1 antibody with human kidney at concentration 3.75 ug/ml.
Immunohistochemistry-Paraffin: Polycystin-1/PKD1 Antibody [NBP1-52552]

Immunohistochemistry-Paraffin: Polycystin-1/PKD1 Antibody [NBP1-52552]

Immunohistochemistry-Paraffin: Polycystin-1/PKD1 Antibody [NBP1-52552] - Analysis of anti-PKD1 antibody with human spleen at concentration 3.75 ug/ml.

Applications for Polycystin-1/PKD1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS (pH 7.3) and 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Polycystin-1/PKD1

Polycystin-1 (also PKD1) is a 500-550 kDa member of the polycystin family of proteins. It is expressed in renal tubule primary cilia, and the membrane region that forms adherens junctions. Polycystin-1 binds to polycystin-2, promoting its insertion into the cell membrane, and regulating its calcium channel activity. In conjunction with polycystin-2, it detects fluid flow and converts this information into calcium signals. It also exists in the ER, where it negatively modulates polycystin-2 mediated calcium release. Mature human polycystin-1 is a 4280 amino acid (aa), 11 transmembrane glycoprotein. Its N-terminal extracellular region (aa 24-3074) is highly modular, and contains a C-type lecin domain, multiple Leu-rich repeats and PKD domains, one GPS region and a PLAT, REJ, WSC and LDLR domain. The cytoplasmic C-terminus (aa 4107-4303) contains a polycystin-2 coiled-coil interaction domain (aa 4220-4251). A percentage of polycystin-1 undergoes autoproteolysis after Leu3048, generating a presumed N-terminal fragment of ~ 350-400 kDa, and a membrane-bound C-terminal fragment of 150 kDa. Over aa 4141-4303, human polycystin-1 shares 71% aa identity with mouse polycystin-1.

Alternate Names

Pc-1, PKD1, Polycystin1, TRPP1

Entrez Gene IDs

5310 (Human)

Gene Symbol

PKD1

UniProt

P98161 (Human)

Additional Polycystin-1/PKD1 Products

Product Documents for Polycystin-1/PKD1 Antibody

Certificate of Analysis

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Product Specific Notices for Polycystin-1/PKD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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