Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Polycystin 2 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human Polycystin 2 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Polycystin 2 Antibody - BSA Free

Western Blot: Polycystin 2 Antibody [NBP3-06363]

Western Blot: Polycystin 2 Antibody [NBP3-06363]

Western Blot: Polycystin 2 Antibody [NBP3-06363] - Anti-PKD2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HEK293 Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDa
Immunohistochemistry: Polycystin 2 Antibody [NBP3-06363]

Immunohistochemistry: Polycystin 2 Antibody [NBP3-06363]

Immunohistochemistry: Polycystin 2 Antibody [NBP3-06363] - Immunochemical staining of human Polycystin 2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: Polycystin 2 Antibody [NBP3-06363]

Immunohistochemistry-Paraffin: Polycystin 2 Antibody [NBP3-06363]

Immunohistochemistry-Paraffin: Polycystin 2 Antibody [NBP3-06363] - Immunochemical staining of human PKD2 in human gallbladder with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: Polycystin 2 Antibody [NBP3-06363]

Immunoprecipitation: Polycystin 2 Antibody [NBP3-06363]

Immunoprecipitation: Polycystin 2 Antibody [NBP3-06363] - PKD2 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-PKD2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-PKD2 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDaObserved band size: 80 kDa

Applications for Polycystin 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Polycystin 2

Polycystin 2 is encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN.

Alternate Names

APKD2, Autosomal dominant polycystic kidney disease type II protein, MGC138466, MGC138468, PC2, Pc-2, PKD4, polycystic kidney disease 2 (autosomal dominant), Polycystic kidney disease 2 protein, polycystin-2, Polycystwin, R48321, transient receptor potential cation channel, subfamily P, member 2, TRPP2

Gene Symbol

PKD2

Additional Polycystin 2 Products

Product Documents for Polycystin 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Polycystin 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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