PON1 Antibody (4X7X2)

Novus Biologicals | Catalog # NBP3-16187

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4X7X2 expressed in HEK293
View all PON1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PON1 (P27169). TGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PON1 Antibody (4X7X2)

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187]

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187]

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: PON1 Antibody (4X7X2) [NBP3-16187]

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187]

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of HepG2 cells, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry-Paraffin: PON1 Antibody (4X7X2) [NBP3-16187]

Immunohistochemistry-Paraffin: PON1 Antibody (4X7X2) [NBP3-16187]

Immunohistochemistry-Paraffin: PON1 Antibody (4X7X2) [NBP3-16187] - Immunohistochemistry of paraffin-embedded human liver using PON1 Rabbit mAb (NBP3-16187) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
PON1 Antibody (4X7X2)

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] -

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Rat liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
PON1 Antibody (4X7X2)

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] -

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Mouse liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
PON1 Antibody (4X7X2)

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] -

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (4X7X2) [NBP3-16187] - Confocal imaging of paraffin-embedded Human liver tissue using PON1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for PON1 Antibody (4X7X2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PON1

Paraoxonase 1 (PON1) is an arylesterase capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. PON1 primarily hydrolyzes paroxon, an organophosphorus anticholinesterase compound produced in vivo by oxidation of the insecticide parathion, to produce p-nitrophenol. Polymorphisms in this gene are a risk factor in coronary artery disease.

Long Name

Paraoxonase 1

Alternate Names

ESA, K-45

Gene Symbol

PON1

Additional PON1 Products

Product Documents for PON1 Antibody (4X7X2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PON1 Antibody (4X7X2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Stem Cell Markers

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Protocols

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FAQs

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