PON2 Antibody
Novus Biologicals | Catalog # NBP1-52005
![Western Blot: PON2 Antibody [NBP1-52005]](https://beta-resources.rndsystems.com/images/products/PON2-Antibody-Western-Blot-NBP1-52005-img0004.jpg "Western Blot: PON2 Antibody [NBP1-52005]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-EKPRARELRIS corresponding to internal region according to NP_000296.2, NP_001018171.1.
Epitope
C-EKPRARELRIS
Reactivity Notes
Expected from sequence similarity: Rat, Porcine, Canine
Specificity
This antibody is expected to recognize isoforms 1 and 2 (NP_000296.2; NP_001018171.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat PON2 Antibody (NBP1-52005) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-PON2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PON2 Antibody
Western Blot: PON2 Antibody [NBP1-52005]
Western Blot: PON2 Antibody [NBP1-52005] - HepG2 lysate (35ug protein in RIPA buffer) using NBP1-52005 at 0.2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: PON2 Antibody [NBP1-52005]](https://beta-resources.rndsystems.com/images/products/PON2-Antibody-Immunohistochemistry-Paraffin-NBP1-52005-img0005.jpg "Immunohistochemistry-Paraffin: PON2 Antibody [NBP1-52005]")
Immunohistochemistry-Paraffin: PON2 Antibody [NBP1-52005]
Immunohistochemistry-Paraffin: PON2 Antibody [NBP1-52005] - 3.75ug/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for PON2 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
0.2 ug/ml
Application Notes
Western blot: Approx 40kDa double band observed in lysates of HepG2 (calculated MW of 39.4kDa according to NP_000296.2 and of 38.0kDa according to NP_001018171.1). Primary incubation was 1 hour.
An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide
IHC: Paraffin embedded Human Placenta.
IHC: Paraffin embedded Human Placenta.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PON2
Long Name
Paraoxonase 2
Alternate Names
A-esterase 2, Serum Aryldialkylphosphatase 2
Gene Symbol
PON2
UniProt
Additional PON2 Products
Product Documents for PON2 Antibody
Product Specific Notices for PON2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PON2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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