POU2F3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83966

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all POU2F3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LESMHTDIKMSGDVADSTDARSTLSQVEPGNDRNGLDFNRQIKTEDLSDSLQQTLSHRPCHLSQGPAMMSGNQMSGLNASPCQDMASLHPLQQLVLVPGHLQSVSQFLLSQTQP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POU2F3 Antibody - BSA Free

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966] - Analysis in human skin and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues
Immunohistochemistry-Frozen: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Frozen: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Frozen: POU2F3 Antibody [NBP1-83966] - Analysis of human airway cell line using POU2F3 antibody. Nuclear staining with DAPI. Image overlap in right panel. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966] - Staining of human skin shows moderate to strong nuclear positivity in keratinocytes.
Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966] - Staining of human skeletal muscle shows no positivity in myocytes
Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966] - Staining of human kidney shows no positivity in cells in tubules.
Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966]

Immunohistochemistry-Paraffin: POU2F3 Antibody [NBP1-83966] - Staining of human pancreas shows no positivity in exocrine glandular cells.
POU2F3 Antibody - BSA Free Western Blot: POU2F3 Antibody - BSA Free [NBP1-83966]

Western Blot: POU2F3 Antibody - BSA Free [NBP1-83966]

Analysis in control (vector only transfected HEK293T lysate) and POU2F3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for POU2F3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Frozen

Validated from a verified customer review.

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-83966 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POU2F3

POU2F3, also referred to as POU domain, class 2, transcription factor 3, is a transcription factor that belongs to the POU protein family. POU2F3 has a 436 amino acid long isoform, a 438 amino acid long isoform, and a 432 amino acid long isoform, all three of which are approximately 47kDa. POU2F3 is highly expressed in keratinocytes and the epidermis. Current research surrounding POU2F3 has shown possible interactions with diseases and disorders such as atherosclerosis, bipolar disorder, herpes, cervical cancer, breast cancer, and neuroendocrine tumors. POU2F3 has also been shown to interact with Bcl6, Ubiquitin and KAT3A/CBP.

Long Name

POU class 2 homeobox 3

Alternate Names

Epoc-1, OCT-11, OCT11, OTF-11, PLA-1, PLA1, Skn-1a

Gene Symbol

POU2F3

Additional POU2F3 Products

Product Documents for POU2F3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for POU2F3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for POU2F3 Antibody - BSA Free

Customer Reviews for POU2F3 Antibody - BSA Free (1)

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Customer Images

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  • POU2F3 Antibody
    Name: avinash waghray
    Application: Immunohistochemistry-Frozen
    Sample Tested: human airway and cell line
    Species: Human
    Verified Customer | Posted 08/23/2019
    Nuclear staining is seen in IF images on right - overlap with DAPI shown on the right
    POU2F3 Antibody - BSA Free NBP1-83966

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Protocols

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