POU3F3 Antibody (CL15299) - BSA Free

Novus Biologicals | Catalog # NBP3-43658

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL15299

Format

BSA Free
View all POU3F3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P20264, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for POU3F3 Antibody (CL15299) - BSA Free

POU3F3 Antibody (CL15299) [Unconjugated] Western blot: POU3F3 Antibody (CL15299) [NBP3-43658]

Western blot: POU3F3 Antibody (CL15299) [NBP3-43658]

Analysis in human cell line HEK 293.
POU3F3 Antibody (CL15299) [Unconjugated] Immunocytochemistry/ Immunofluorescence : POU3F3 Antibody (CL15299) [NBP3-43658]

Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody (CL15299) [NBP3-43658]

Staining in SH-SY5Y cell line with POU3F3 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Applications for POU3F3 Antibody (CL15299) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POU3F3

POU3F3, also known as POU Class 3 Homeobox 3, is a transcription factor that is primarily expressed in the brain and CNS. POU3F3 interacts with SOX4 and SOX11, and is implicated in the development of neurons. Current research surrounding POU3F3 has shown potential linkages with neuronitis and cerebritis. POU3F3 has also been shown to interact with POU3F4, CAV1, SOX10, and GADD34.

Alternate Names

Brain-1, Brain-specific homeobox/POU domain protein 1, Brn-1, BRN1brn-1, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, OTF-8, OTF8oct-8, POU class 3 homeobox 3, POU domain class 3, transcription factor 3, POU domain, class 3, transcription factor 3

Gene Symbol

POU3F3

Additional POU3F3 Products

Product Documents for POU3F3 Antibody (CL15299) - BSA Free

Certificate of Analysis

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Product Specific Notices for POU3F3 Antibody (CL15299) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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