PP1 alpha/PPP1A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38559

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PP1 alpha Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: QILKPADKNKGKYGQFSGLNPGGRPIT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP1 alpha/PPP1A Antibody - BSA Free

Western Blot: PP1 alpha/PPP1A Antibody [NBP2-38559]

Western Blot: PP1 alpha/PPP1A Antibody [NBP2-38559]

Western Blot: PP1 alpha/PPP1A Antibody [NBP2-38559] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/ Immunofluorescence: PP1 alpha/PPP1A Antibody [NBP2-38559]

Immunocytochemistry/ Immunofluorescence: PP1 alpha/PPP1A Antibody [NBP2-38559]

Immunocytochemistry/Immunofluorescence: PP1 alpha/PPP1A Antibody [NBP2-38559] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody [NBP2-38559]

Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody [NBP2-38559]

Immunohistochemistry-Paraffin: PP1 alpha/PPP1A Antibody [NBP2-38559] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Applications for PP1 alpha/PPP1A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP1 alpha

Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favouring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).

Long Name

Protein Phosphatase 1 Catalytic Subunit alpha

Alternate Names

PPP1A, PPP1CA

Gene Symbol

PPP1CA

UniProt

P62136 (Human)

Additional PP1 alpha Products

Product Documents for PP1 alpha/PPP1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PP1 alpha/PPP1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PP1 alpha/PPP1A Antibody - BSA Free

There are currently no reviews for this product. Be the first to review PP1 alpha/PPP1A Antibody - BSA Free and earn rewards!

Have you used PP1 alpha/PPP1A Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies