PP2C gamma/PPM1G Antibody
Novus Biologicals | Catalog # NB110-38867
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 400 and 450 of human Protein Phosphatase Magnesium-dependent 1 Gamma using the numbering given in entry NP_817092.1 (GeneID 5496).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PP2C gamma/PPM1G Antibody
Western Blot: PP2C gamma/PPM1G Antibody [NB110-38867]
Western Blot: PP2C gamma/PPM1G Antibody [NB110-38867] - Detection of Human PPM1G on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T (50 microgram) using NB110-38867. PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody NB110-38869.Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867]
Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867] - Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DABImmunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867]
Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-PPM1G used at a dilution of 1:500.Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867]
Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-PPM1G used at a dilution of 1:100.Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867]
Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867] - Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DABApplications for PP2C gamma/PPM1G Antibody
Application
Recommended Usage
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
This antibody can be used for immunoprecipitation and Western blot analysis. Prepare working dilution immediately before use.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PP2C gamma
Long Name
Protein Phosphatase 2C gamma
Alternate Names
PP2CG, PPM1C, PPM1G, PPP2CG
Entrez Gene IDs
5496 (Human)
Gene Symbol
PPM1G
UniProt
Additional PP2C gamma Products
Product Documents for PP2C gamma/PPM1G Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PP2C gamma/PPM1G Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for PP2C gamma/PPM1G Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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