PPAR delta/NR1C2 Antibody - N-terminal - BSA Free

Novus Biologicals | Catalog # NB600-637

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPAR delta/NR1C2 Primary Antibodies »

Product Specifications

Immunogen

PPAR delta/NR1C2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse PPAR delta/NR1C2. (Uniprot: P35396)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from mouse and rat sources based on 100% homology for the immunogen sequence. Cross-reactivity with PPAR delta protein from human, chimpanzee and rabbit may occur as this sequence shows 85% homology with the protein from these sources. Cross-reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.

Localization

Nuclear

Specificity

No reactivity is expected against other subtypes of PPAR.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity purified antibody is directed against mouse PPAR delta/NR1C2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for PPAR delta/NR1C2 Antibody - N-terminal - BSA Free

Western Blot: PPAR delta/NR1C2 AntibodyN-terminal [NB600-637]

Western Blot: PPAR delta/NR1C2 AntibodyN-terminal [NB600-637]

Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Immunohistochemistry: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637]

Immunohistochemistry: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637]

Immunohistochemistry: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Immunohistochemistry of PPAR delta/NR1C2/NR1C2 antibody. Antigen Retrieval: HIER pH 6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta/NR1C2 in human myocardiocitys at 1:500.
ELISA: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637]

ELISA: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637]

ELISA: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - ELISA results of purified PPAR delta/NR1C2 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.
PPAR delta/NR1C2 Antibody - N-terminal

PPAR delta/NR1C2 Antibody - N-terminal

Immunohistochemistry of Rabbit Anti-PPAR delta (N terminal specific). Antigen Retrieval: HIER pH 6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta in human myocardiocitys at 1:500.
PPAR delta/NR1C2 Antibody - N-terminal

PPAR delta/NR1C2 Antibody - N-terminal

Affinity Purified Rabbit Anti-PPAR delta is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta present in mouse heart whole cell lysates

Applications for PPAR delta/NR1C2 Antibody - N-terminal - BSA Free

Application
Recommended Usage

ELISA

1:30000-1:70000

Immunohistochemistry

1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:5000
Application Notes
This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 43 kDa in size corresponding to PPAR delta by western blot in the appropriate tissue or cell lysate.

IHC-P use reported by customer in Gerbil stomach tissue.

Reviewed Applications

Read 1 review rated 4 using NB600-637 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: PPAR delta/NR1C2

The Peroxisome Proliferator Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. There are 3 known subtypes of PPARs, PPAR alpha, PPAR delta and PPAR gamma. PPAR gamma is believed to be involved in adipocyte differentiation.

Long Name

Peroxisome Proliferator-activated Receptor delta

Alternate Names

NR1C2, NUC1, PPARD

Entrez Gene IDs

5467 (Human); 19015 (Mouse); 25682 (Rat)

Gene Symbol

PPARD

UniProt

P35396 (Mouse)

Additional PPAR delta/NR1C2 Products

Product Documents for PPAR delta/NR1C2 Antibody - N-terminal - BSA Free

Certificate of Analysis

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Product Specific Notices for PPAR delta/NR1C2 Antibody - N-terminal - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PPAR delta/NR1C2 Antibody - N-terminal - BSA Free

Customer Reviews for PPAR delta/NR1C2 Antibody - N-terminal - BSA Free (1)

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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Gerbil stomach
    Species: Other
    Verified Customer | Posted 01/28/2010

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