PPAR gamma/NR1C3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-59223

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPAR gamma/NR1C3 Primary Antibodies »

Product Specifications

Immunogen

The exact immunogen is propietary information.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPAR gamma/NR1C3 Antibody - BSA Free

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-59223]

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-59223]

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-59223] - The antibody was diluted 1:2,000 in TBS-Tween containing 3% skimmed milk. Figure shows the result of 293T cells transfected with pNTAP-PPARG (lane 1) and of non-transfected cells (lane 2). The position of the protein of interest is indicated on the right the marker (kDa) is shown on the left.
ELISA: PPAR gamma/NR1C3 Antibody [NBP2-59223]

ELISA: PPAR gamma/NR1C3 Antibody [NBP2-59223]

ELISA: PPAR gamma/NR1C3 Antibody [NBP2-59223] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PPARG. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:70,250

Applications for PPAR gamma/NR1C3 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1 ug

ELISA

1:1000

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide and 0.05% ProClin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPAR gamma/NR1C3

PPAR gamma(PPARG), which encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Long Name

Peroxisome Proliferator-activated Receptor gamma

Alternate Names

NR1C3, PPARG

Gene Symbol

PPARG

Additional PPAR gamma/NR1C3 Products

Product Documents for PPAR gamma/NR1C3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PPAR gamma/NR1C3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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