PPOX Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80885

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPOX Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ETHLTSRGVSVLRGQPVCGLSLQAEGRWKVSLRDSSLEADHVISAIPASVLSELLPAEAAPLARALSAITAVSVAVVNLQYQGAH

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPOX Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PPOX Antibody [NBP1-80885]

Immunocytochemistry/ Immunofluorescence: PPOX Antibody [NBP1-80885]

Immunocytochemistry/Immunofluorescence: PPOX Antibody [NBP1-80885] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & mitochondria.
Immunohistochemistry: PPOX Antibody [NBP1-80885]

Immunohistochemistry: PPOX Antibody [NBP1-80885]

Immunohistochemistry: PPOX Antibody [NBP1-80885] - Staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in glandular cells.

Applications for PPOX Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPOX

PPOX encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.

Alternate Names

EC 1.3.3.4, MGC8485, PPOVP, protoporphyrinogen oxidase, V290M, variegate porphyria

Entrez Gene IDs

5498 (Human)

Gene Symbol

PPOX

UniProt

P50336 (Human)

Additional PPOX Products

Product Documents for PPOX Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PPOX Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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