PPP3CB Antibody (PSH0-21)

Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848]

Western Blot: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Jurkat cell lysate
Lane 3: Rat brain tissue lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 59 kDa
Observed band size: 59 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32848) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PPP3CB Antibody (PSH0-21) [NBP3-32848]

Immunohistochemistry: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-PPP3CB antibody at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: PPP3CB Antibody (PSH0-21) [NBP3-32848]

Immunocytochemistry/ Immunofluorescence: PPP3CB Antibody (PSH0-21) [NBP3-32848] - Immunocytochemistry analysis of MCF-7 cells labeling PPP3CB with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP3CB antibody (NBP3-32848) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.