PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20139

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Mass Spectrometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # RAB-C367

Format

Azide and BSA Free
View all PRDM4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRDM4 protein under non-denaturing conditions.

Epitope

This antibody binds to a folded domain, amino acids 390-540.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free

PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free [NBP3-20139] -

Immunocytochemistry/Immunofluorescence: PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free [NBP3-20139] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C367 (NBP3-20139) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20139, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free [NBP3-20139] -

Immunocytochemistry/Immunofluorescence: PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free [NBP3-20139] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C367 (NBP3-20139) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20139, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Mass Spectrometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: PRDM4

PRDM4/PFM1 may function as a transcription factor involved in cell differentiation. It contains 6 C2H2-type zinc fingers and is expressed in many tissues. Highly expressed in ovary, testis, pancreas, brain, heart and prostate.

Alternate Names

MGC45046, PFM1PR domain-containing protein 4, PR domain containing 4, PR domain zinc finger protein 4, PR-domain zinc-finger protein PFM1

Gene Symbol

PRDM4

Additional PRDM4 Products

Product Documents for PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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