PRMT1 Antibody (10W10U4)

Novus Biologicals | Catalog # NBP3-16423

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10W10U4 expressed in HEK293
View all PRMT1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRMT1 (Q99873). MAAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRMT1 Antibody (10W10U4)

Western Blot: PRMT1 Antibody (10W10U4) [NBP3-16423]

Western Blot: PRMT1 Antibody (10W10U4) [NBP3-16423]

Western Blot: PRMT1 Antibody (10W10U4) [NBP3-16423] - Western blot analysis of extracts of various cell lines, using PRMT1 Rabbit mAb (NBP3-16423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: PRMT1 Antibody (10W10U4) [NBP3-16423]

Immunocytochemistry/ Immunofluorescence: PRMT1 Antibody (10W10U4) [NBP3-16423]

Immunocytochemistry/Immunofluorescence: PRMT1 Antibody (10W10U4) [NBP3-16423] - Immunofluorescence analysis of U-2 OS cells using PRMT1 Rabbit mAb (NBP3-16423) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PRMT1 Antibody (10W10U4) [NBP3-16423]

Immunohistochemistry-Paraffin: PRMT1 Antibody (10W10U4) [NBP3-16423]

Immunohistochemistry-Paraffin: PRMT1 Antibody (10W10U4) [NBP3-16423] - Immunohistochemistry of paraffin-embedded human colon using PRMT1 Rabbit mAb (NBP3-16423) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: PRMT1 Antibody (10W10U4) [NBP3-16423]

Immunocytochemistry/ Immunofluorescence: PRMT1 Antibody (10W10U4) [NBP3-16423]

Immunocytochemistry/Immunofluorescence: PRMT1 Antibody (10W10U4) [NBP3-16423] - Immunofluorescence analysis of C6 cells using PRMT1 Rabbit mAb (NBP3-16423) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: PRMT1 Antibody (10W10U4) [NBP3-16423]

Immunocytochemistry/ Immunofluorescence: PRMT1 Antibody (10W10U4) [NBP3-16423]

Immunocytochemistry/Immunofluorescence: PRMT1 Antibody (10W10U4) [NBP3-16423] - Immunofluorescence analysis of NIH-3T3 cells using PRMT1 Rabbit mAb (NBP3-16423) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PRMT1 Antibody (10W10U4) [NBP3-16423]

Immunohistochemistry-Paraffin: PRMT1 Antibody (10W10U4) [NBP3-16423]

Immunohistochemistry-Paraffin: PRMT1 Antibody (10W10U4) [NBP3-16423] - Immunohistochemistry of paraffin-embedded rat brain using PRMT1 Rabbit mAb (NBP3-16423) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
PRMT1 Antibody (10W10U4)

Western Blot: PRMT1 Antibody (10W10U4) [NBP3-16423] -

Western Blot: PRMT1 Antibody (10W10U4) [NBP3-16423] - Western blot analysis of various lysates using PRMT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for PRMT1 Antibody (10W10U4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRMT1

PRMTs are a family of proteins that either monomethylate or dimethylate the guanidino nitrogen atoms of arginine side chains. This common post-translational modification is implicated in protein trafficking, signal transduction, and transcriptional regulation. Most methyltransferases use the methyl donor, S-adenosyl-L-methionine (AdoMet), as a cofactor.

Long Name

Protein Arginine Methyltransferase 1

Alternate Names

ANM1, HCP1, HMT1, HMT2, HRMT1L2, IR1B4

Gene Symbol

PRMT1

Additional PRMT1 Products

Product Documents for PRMT1 Antibody (10W10U4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PRMT1 Antibody (10W10U4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Epigenetics

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Protocols

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