PRMT1 Antibody (779) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43744

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 779

Format

Azide and BSA Free
View all PRMT1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PRMT1. The exact sequence is proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for PRMT1 Antibody (779) - Azide and BSA Free

Western Blot: PRMT1 Antibody (779) [NBP2-43744]

Western Blot: PRMT1 Antibody (779) [NBP2-43744]

Western Blot: PRMT1 Antibody (779) [NBP2-43744] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PRMT1 Antibody (779) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PRMT1 Antibody (779) [NBP2-43744]

Immunohistochemistry-Paraffin: PRMT1 Antibody (779) [NBP2-43744]

Immunohistochemistry-Paraffin: PRMT1 Antibody (779) [NBP2-43744] - PRMT1 Antibody (779) detects PRMT1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. PRMT1 stained by PRMT1 Antibody (779) diluted at 1:200.
Western Blot: PRMT1 Antibody (779) [NBP2-43744]

Western Blot: PRMT1 Antibody (779) [NBP2-43744]

Western Blot: PRMT1 Antibody (779) [NBP2-43744] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE PRMT1 antibody [779] dilution: 1:500
Immunohistochemistry-Paraffin: PRMT1 Antibody (779) [NBP2-43744]

Immunohistochemistry-Paraffin: PRMT1 Antibody (779) [NBP2-43744]

Immunohistochemistry-Paraffin: PRMT1 Antibody (779) [NBP2-43744] - PRMT1 Antibody (779) detects PRMT1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. PRMT1 stained by PRMT1 Antibody (779) diluted at 1:200.
PRMT1 Antibody (779) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: PRMT1 Antibody (779) [NBP2-43744] -

PRMT1 antibody [GT779] detects PRMT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRMT1 protein stained by PRMT1 antibody [GT779] (NBP2-43744) diluted at 1:200.
Blue: Hoechst 33342 staining.
PRMT1 Antibody (779) - Azide and BSA Free

Western Blot: PRMT1 Antibody (779) [NBP2-43744] -

PRMT1 antibody [GT779] detects PRMT1 protein by western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug NIH-3T3 whole cell lysate/extract
D. 30 ug Raw264.7 whole cell lysate/extract
E. 30 ug C2C12 whole cell lysate/extract
10% SDS-PAGE
PRMT1 antibody [GT779] (NBP2-43744) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for PRMT1 Antibody (779) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRMT1

PRMTs are a family of proteins that either monomethylate or dimethylate the guanidino nitrogen atoms of arginine side chains. This common post-translational modification is implicated in protein trafficking, signal transduction, and transcriptional regulation. Most methyltransferases use the methyl donor, S-adenosyl-L-methionine (AdoMet), as a cofactor.

Long Name

Protein Arginine Methyltransferase 1

Alternate Names

ANM1, HCP1, HMT1, HMT2, HRMT1L2, IR1B4

Gene Symbol

PRMT1

Additional PRMT1 Products

Product Documents for PRMT1 Antibody (779) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PRMT1 Antibody (779) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Epigenetics

Citations for PRMT1 Antibody (779) - Azide and BSA Free

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Protocols

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FAQs

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