PRMT5 Antibody (7K2G5)

Novus Biologicals | Catalog # NBP3-15758

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7K2G5 expressed in HEK293
View all PRMT5 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRMT5 (O14744). MAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFIQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLSPWIRPDSKVEKIR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRMT5 Antibody (7K2G5)

Western Blot: PRMT5 Antibody (7K2G5) [NBP3-15758]

Western Blot: PRMT5 Antibody (7K2G5) [NBP3-15758]

Western Blot: PRMT5 Antibody (7K2G5) [NBP3-15758] - Western blot analysis of extracts of various cell lines, using PRMT5 antibody (NBP3-15758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758]

Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758]

Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758] - Immunohistochemistry of paraffin-embedded mouse testis using PRMT5 antibody (NBP3-15758) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758]

Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758]

Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758] - Immunohistochemistry of paraffin-embedded rat testis using PRMT5 antibody (NBP3-15758) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for PRMT5 Antibody (7K2G5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRMT5

Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

Long Name

Protein arginine N-methyltransferase 5

Alternate Names

EC 2.1.1.320, HRMT1L5, IBP72, JBP1, SKB1, SKB1 homolog

Gene Symbol

PRMT5

Additional PRMT5 Products

Product Documents for PRMT5 Antibody (7K2G5)

Certificate of Analysis

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Product Specific Notices for PRMT5 Antibody (7K2G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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