Proapoptotic Caspase Adaptor Protein Antibody
Novus Biologicals | Catalog # NBP1-31619
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Rhesus Macaque (97%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Proapoptotic Caspase Adaptor Protein. The exact sequence is proprietary.
Localization
Secreted (Potential), Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Proapoptotic Caspase Adaptor Protein Antibody
Western Blot: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619]
Western Blot: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PACAP antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Immunohistochemistry-Paraffin: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619]
Immunohistochemistry-Paraffin: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] - Paraffin-embedded normal colon mucosa with lymphocyte. PACAP antibody [N1C3] dilution: 1:500.Immunohistochemistry-Paraffin: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] -
Immunohistochemistry-Paraffin: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] - Proapoptotic Caspase Adaptor Protein antibody [N1C3] detects Proapoptotic Caspase Adaptor Protein protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.
Proapoptotic Caspase Adaptor Protein stained by Proapoptotic Caspase Adaptor Protein antibody [N1C3] (NBP1-31619) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] -
Immunohistochemistry-Paraffin: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] - Proapoptotic Caspase Adaptor Protein antibody [N1C3] detects Proapoptotic Caspase Adaptor Protein protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.
Proapoptotic Caspase Adaptor Protein stained by Proapoptotic Caspase Adaptor Protein antibody [N1C3] (NBP1-31619) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] -
Immunocytochemistry/ Immunofluorescence: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] - Proapoptotic Caspase Adaptor Protein antibody [N1C3] detects Proapoptotic Caspase Adaptor Protein protein at cytoplasm by immunofluorescent analysis.Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Proapoptotic Caspase Adaptor Protein stained by Proapoptotic Caspase Adaptor Protein antibody [N1C3] (NBP1-31619) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Western Blot: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] -
Western Blot: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] - Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PACAP antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Frozen: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-31619] -
Proapoptotic Caspase Adaptor Protein antibody [N1C3] detects Proapoptotic Caspase Adaptor Protein protein at cytoplasm in mouse brain by immunohistochemical analysis.Sample:Frozen section of mouse brain.
Proapoptotic Caspase Adaptor Protein antibody [N1C3] (NBP1-31619) diluted at 1:500.
Applications for Proapoptotic Caspase Adaptor Protein Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Proapoptotic Caspase Adaptor Protein
Alternate Names
caspase-2 binding protein, FLJ32987, HSPC190, marginal zone B and B1 cell-specific protein, MGC29506, PACAP, pERp1, plasma cell-induced ER protein 1, plasma cell-induced resident endoplasmic reticulum protein, Plasma cell-induced resident ER protein, Proapoptotic caspase adapter protein, proapoptotic caspase adaptor protein
Gene Symbol
MZB1
Additional Proapoptotic Caspase Adaptor Protein Products
Product Documents for Proapoptotic Caspase Adaptor Protein Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Proapoptotic Caspase Adaptor Protein Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for Proapoptotic Caspase Adaptor Protein Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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